The 46-year-old woman charged with two counts of DUI had her first court appearance Monday morning in St. Johns County.
Kim Johnston, is being held on a $100,000 bond in a St. Johns County jail.
Due to the severity of the crash, Johnston could face manslaughter.
RELATED: JSO bailiff hit by DUI driver has died, per Jacksonville Sheriff's Office
Florida Highway Patrol said Johnston's car hit two Jacksonville Sheriff's Office employees early Sunday morning while they were traveling on I-95, causing them to lose control and overturn.
The two employees -- Cathy Adams, a JSO bailiff, and her husband William "Jack" Adams, a JSO officer were reportedly traveling back from the state championship game, where the Mandarin Mustangs claimed a state title in football.
Their two teenagers were also in the car.
Johnston reportedly refused to blow for a breathalyzer test.
[ Want to help? Here's how you can donate to the Adams family ]
JSO announced Sunday night that Cathy Adams died of her injuries. She was 43.
The Facebook page of Oakleaf Family Chiropractic mentioned the crash, stating:
"To our patients, our friends and family:
We at Oakleaf Chiropractic and Injury Center (formerly Oakleaf Family Chiropractic) regretfully must say that a member of our staff has made a terrible choice during her personal time that led to a horrific crash and loss of life. She had recently joined us just a few weeks ago, and we conduct an extensive criminal background check on all employees, and she previously has been a respected member of the chiropractic community throughout Jacksonville for many years.
Our deepest condolences to the victim's family, and the wider JSO family who has suffered from this loss. God Bless."